BISBENZIMIDE H 33342 TRIHYDROCHLORIDE
Hoechst 33342 (trihydrochloride)
CAS: 875756-97-1
Molecular Formula: C27H31Cl3N6O
BISBENZIMIDE H 33342 TRIHYDROCHLORIDE - Names and Identifiers
BISBENZIMIDE H 33342 TRIHYDROCHLORIDE - Physico-chemical Properties
| Molecular Formula | C27H31Cl3N6O |
| Molar Mass | 561.93364 |
| Solubility | DMSO: ≥ 46 mg/mL; H2O: ≥ 5.6 mg/mL |
| Color | Yellow to green powder or solid |
| Maximum wavelength(λmax) | ['350 nm (H2O/DNA); 357 nm\n(MeOH/H+)'] |
| pKa | 11.08 ± 0.69, most acidic, temperature:25 °C; 7.66 ± 0.42, most basic(at 25℃) |
| Storage Condition | Inert atmosphere,Store in freezer, under -20°C |
| In vitro study | Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI. |
BISBENZIMIDE H 33342 TRIHYDROCHLORIDE - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 1.78 ml | 8.898 ml | 17.796 ml |
| 5 mM | 0.356 ml | 1.78 ml | 3.559 ml |
| 10 mM | 0.178 ml | 0.89 ml | 1.78 ml |
| 5 mM | 0.036 ml | 0.178 ml | 0.356 ml |
Last Update：2024-01-02 23:10:35
BISBENZIMIDE H 33342 TRIHYDROCHLORIDE - Reference Information
| Biological applications | detectingβ-amyloid (Aβ) plaques; detecting/measuring organizations; identifying/counting cells; studying multidrug resistance; aptopsia; cell cycle |
| Main application | Photoresists |
| biological activity | Hoechst 33342 trihydro chloride is a DNA dye that can penetrate cell membranes and fluoresce blue. |
| Target | Dye reagent DNA Stain |
Last Update：2024-04-09 20:52:54
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Product Name: Hoechst 33342 (trihydrochloride) Visit Supplier Webpage Request for quotationCAS: 875756-97-1
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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